At the upcoming BioProcess International European Summit, Deborah Hol, PhD Senior Research Scientist Upstream Process Development at Synthon Biopharmaceuticals BV, is speaking on how to select the most suitable media for your cells. We caught up with Deborah for an exclusive interview on a recent study she conducted:
“Many different media are available to culture CHO cells. Most are either growth supporting or productivity supporting. Ideally, the basal medium is a growth supporting medium, while the feed medium is supporting both growth and productivity.
Vendors have different philosophies when developing a medium, namely rich or lean basal media in combination with rich or lean feeds. Feed media can contain similar ingredients as their basal media or only a certain group of nutrients. Different types of CHO cells have different nutrient requirements, and different clones from the same host may have different nutrient requirements. This makes choosing a medium a complex process – which type of basal media to choose and is the connected feed medium the best choice to use as a feed?
In the study we conducted, two clones producing the same mAb are compared on their response to a range of basal media. Clone I is fast-growing and low-producing, while Clone II is slower-growing, but high-producing. A panel of eleven different basal media from different vendors was evaluated for growth, titer and metabolism.
From this data, media supporting cell growth and media supporting mAb productivity can be identified. By combining media that supports growth or supports production, an optimal (feed) media system can be designed.
During my presentation, an overview will given on the response of the cells to the different media, alongside an example of the added value of combining two media.”
Deborah Hol’s talk – Cell Culture media and Feed Optimisation to Enhance Product Quality and Productivity – is at 14:05 on Day 2 of BioProcess International European Summit. The conference is running from 11th-14th April in Vienna – register your place here.