Host cell protein and residual Protein A quantitation using qPCR

An upcoming webinar with Thermo Fisher Scientific

About this event:

As the global development of bio-therapeutics continues to rise, the rigorous characterization of process impurities and protein contaminants is becoming ever more critical. We have developed a novel qPCR-based ultra-high performance assay platform that proves to be an excellent overall solution for process analytics, particularly in the routine testing and accurate quantitation of host cell proteins and residual protein A. It offers sensitivity levels far surpassing that of any other immunoassay, with up to 5 logs of dynamic range. It retains excellent dilutional linearity and recovery throughout the entire assay range, with single-digit %CV assay precision. The Applied Biosystems™ ProteinSEQTM system is a content-agnostic assay platform that enables easy customization to detect and quantitate any protein impurity in bioprocessing and bioproduction. This study demonstrates the high performance quantitation of host cell proteins using an industry-standard antibody from the leading HCP vendor, and the ultra-high sensitivity quantitation of residual Protein A. Fully supported by instrument hardware with automated sample processing and qPCR reaction and readings, the ProteinSEQ system provides the best comprehensive assay solution for bioprocess analytics.

In this webinar we’ll discuss:

  • The critical aspect of contaminant testing in bioprocessing
  • What is the ProteinSEQ system and why it is the best overall solution for process analytics
  • Data comparison between the ProteinSEQ system and commercial ELISA assays for HCP
  • Why the ProteinSEQ system is an ideal assay platform with which to streamline laboratory workflow

About the presenter:
darrick-dayneDarick Dayne, Ph.D. is the Senior Product Manager in the Pharma Analytics group at ThermoFisher Scientific. He brings a wealth of experience in developing novel analytical tools and successfully bringing them to market. His deep expertise in biopharma analytical solutions spans from early stage discovery to final product manufacturing and QC. He received his MS degree in physical chemistry from Northwestern University, and his PhD in biophysics from the Max Planck Institute in Germany.

Register here.

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